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稻虱缨小蜂Anagrus nilaparvatae Pang et Wang是控制稻飞虱种群暴发的主要寄生性天敌之一,高温是制约其种群动态的重要因子。为了解35℃高温驯化获得的稻虱缨小蜂耐热品系(NR品系)的生防潜力,对不同温度下该品系的发育历期、有效积温、寄主卵选择、产卵量以及极端高温胁迫参数进行了测定。结果发现,与未经高温驯化的稻虱缨小蜂品系(HZ品系)相比,20℃时,NR品系雄蜂的发育速率显著高于HZ品系,这一差异主要表现在卵-幼虫中期和蛹期的发育时间明显缩短。NR品系在高温下能维持正常的产卵能力,35℃下NR品系产卵量仍能达到18.80粒/雌。NR品系有更强的耐极端温度的能力,50℃下NR品系雄蜂的热击倒时间为15.19 min,比HZ品系雄蜂长3.03 min。补充不同营养对NR品系的产卵量存在影响,35℃条件下补充10%蔗糖既能延长其寿命,也能提高产卵量。以上研究结果表明高温驯化得到的稻虱缨小蜂品系在35℃高温环境中能维持正常的生长发育和寄生能力,具备适应稻田夏季高温环境的潜力。 相似文献
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The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine. 相似文献
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8株优良大豆根瘤菌与不同地区27个大豆主栽品种的匹配性研究 总被引:2,自引:0,他引:2
大豆是我国重要的油料作物和高蛋白粮饲兼用作物,具有与根瘤菌共生固氮的能力。近十几年来,我国大豆品种更新快,导致大豆根瘤菌株与新品种匹配能力差、接种效果不明显。筛选与主栽大豆品种匹配性好、固氮效率高的广谱性优良菌株,可为针对性的施用大豆根瘤菌接种剂提供菌种资源和方案。选取本实验室前期分离保存的6个优良快生型大豆根瘤菌株和2个慢生型大豆根瘤菌株,在砂培盆栽条件下与不同地区的27个大豆主栽品种进行匹配试验。测定分析了植株地上部分生物量、高度、根瘤数量、根瘤生物量和根瘤固氮酶活指标。结果表明:不同大豆根瘤菌之间结瘤固氮能力存在极显著的差异;供试根瘤菌均能够与国内24个大豆品种结瘤,广谱性较好;植株地上部分高度、根瘤数量、根瘤生物量和根瘤固氮酶活与地上部分生物量呈显著相关;大部分接种根瘤菌后的植物生物量显著高于CK;HN01、GR3、HH29、HH103匹配性和固氮效率均不逊色于USDA110,具有在东北地区、黄淮海地区、长江流域、东南地区推广的潜能;从品种来看,中豆39、BD2、天隆1号与8株供试大豆根瘤菌的匹配接种表现出高生物量特点。此外,本文还筛选出大豆-大豆根瘤菌的表型最佳匹配组合中豆39-GR3,适合长江流域地区;同样筛选到东北地区、黄淮海地区、东南地区最佳匹配组合,分别是HN01-辽豆14、HN01-徐豆14、HN01-BD2。本文初步建立了优良根瘤菌与大豆主栽品种的匹配关系,为在田间试验中进一步筛选和应用这些优良菌株提供了材料和指导。 相似文献
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Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens 下载免费PDF全文
ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300
We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control. 相似文献
6.
Goatpox (GTP), sheeppox (SPP) and lumpy skin disease (LSD) are three severe diseases of goat, sheep and cattle. Their typical clinical symptoms are characterized by vesicles, papules, nodules, pustules and scabs on animal skins. The GTP, SPP and LSD are caused by goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV), respectively, all of which belong to the genus Capripoxvirus in the family Poxviridae. Several capripoxvirus (CaPV) isolates have been virulently attenuated through serial passaging in vitro for production of live vaccines. CaPV-based vector systems have been broadly used to construct recombinant vaccines for delivering foreign antigens, many of which have been demonstrated to induce effective immune protections. Homologous recombination is the most commonly used method for constructing recombinant CaPVs. Here, we described a methodology for generation of recombinant CaPVs by the homologous recombination, and further reviewed CaPV-vectored vaccines for delivering foreign antigens. 相似文献
7.
羊源链球菌的分群鉴定及多价灭活苗的研制 总被引:1,自引:0,他引:1
2000~2003年,从安阳、邯郸等6个地区采取280份羊的病料,从中分离到150株链球菌,对其中20株典型分离株进行了生化分群鉴定及兰氏分群A~G诊断试剂鉴定.结果表明,6个地区发生的羊的链球菌病主要由C群兽疫链球菌(7/20),D群(8/20),E群(2/20)引起.其分布无明显地域性.选择C、D、E群中各1株为菌种,制备多价链球菌灭活苗,通过免疫试验,保护率达92%以上,免疫期半年以上,疫苗4℃保存期1年以上,证明该灭活苗安全性好,免疫效果明显. 相似文献
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